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本公司產(chǎn)品僅供科研使用,不用于人體及臨床診斷

小鼠促黃體激素(LH)ELISA檢測(cè)試劑盒

 一鍵復(fù)制產(chǎn)品信息
英文名稱: Mouse?Luteinizing?Hormone (LH) ELISA Kit
實(shí)驗(yàn)類型: 競(jìng)爭(zhēng)法
檢測(cè)范圍: 1.56-100mIU/mL(特殊要求可定制)
靈敏度: 0.75mIU/mL
種屬: 小鼠
保存溫度: 2-8℃
樣本類型: 血清、血漿 和 其他生物液體
產(chǎn)品說明書
品牌 貨號(hào) 純度/規(guī)格 包裝 市場(chǎng)價(jià) 會(huì)員價(jià)格 庫存 數(shù)量 購物車
江萊生物 JL10432-48T 48T 1盒 ¥1400.00
江萊生物 JL10432-96T 96T 1盒 ¥2300.00
江萊生物 JL10432-5×96T 5×96T 1盒 ¥9775.00

|   別稱

 

ICSH; Lutropin; Interstitial Cell Stimulating Hormone

 

 

|   LH 簡(jiǎn)介

 

黃體生成素(LH,也稱為促性腺激素,有時(shí)也稱為促性腺激素)是由垂體前葉的促性腺激素細(xì)胞產(chǎn)生的一種激素。它的產(chǎn)生受來自下丘腦的促性腺激素釋放激素(GnRH)的調(diào)節(jié)。LH是一種異型二聚體糖蛋白,每個(gè)單體單位是一個(gè)糖蛋白分子,一個(gè)α亞單位和一個(gè)β亞單位組成完整的功能性蛋白。

 

 

|   特異性

 

可檢測(cè)樣本中小鼠的LH,且與其類似物無明顯交叉反應(yīng)

 

 

|   典型數(shù)據(jù)及參考曲線

 

由于實(shí)驗(yàn)操作條件的不同( 如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等),標(biāo)準(zhǔn)曲線的OD值會(huì)有所差異。以下數(shù)據(jù)和曲線僅供參考,實(shí)驗(yàn)者需根據(jù)自己的實(shí)驗(yàn)建立標(biāo)準(zhǔn)曲線。

 

濃度(mIU/mL) 100 50 25 12.5 6.25 3.12 1.56 0
OD值 2.28 1.85 1.23 0.8 0.53 0.39 0.3 0.08
校正OD值 2.2 1.77 1.15 0.72 0.45 0.31 0.22 -

 

注意:本圖僅供參考,應(yīng)以同次試驗(yàn)標(biāo)準(zhǔn)品所繪標(biāo)準(zhǔn)曲線計(jì)算標(biāo)本含量。

 

 

|   重復(fù)性

 

板內(nèi)變異系數(shù)小于10%,板間變異系數(shù)小于10%

 

 

|   回收率

 

在選取的健康小鼠血清、血漿中加入3個(gè)不同濃度水平的小鼠LH,計(jì)算回收率

 

樣本類型 范圍 平均回收率
血清(n=8) 84-101 96
血漿(n=8) 92-105 102

 

 

|   線性稀釋

 

分別在選取的4份健康小鼠血清、血漿中加入高濃度小鼠LH,在標(biāo)準(zhǔn)曲線動(dòng)力學(xué)范圍內(nèi)進(jìn)行稀釋,評(píng)估線性。

 

稀釋比例 回收率(%) 血清 血漿
1:2 范圍(%) 85-95 88-96
平均回收率(%) 91 93
1:4 范圍(%) 89-103 87-108
平均回收率(%) 94 98

 

 

注:ELISA試劑盒檢測(cè)范圍等數(shù)據(jù),因檢測(cè)樣本 的不同而調(diào)整,實(shí)際數(shù)據(jù)以隨貨說明書為準(zhǔn)。

 

 

|   ELISA操作視頻

 

ELISA操作視頻        ELISA保存溫度

 

 

|   ELISA操作前必讀 / 下載

 

樣本處理    血液樣本    加樣    溫育

 

洗板    擬合曲線    常見問題    下載

 

 

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