|   別稱

DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

|   TNF-α 簡介

腫瘤壞死因子α（TNF-α）由中性粒細(xì)胞、活化的淋巴細(xì)胞、巨噬細(xì)胞、NK細(xì)胞、LAK細(xì)胞、星形膠質(zhì)細(xì)胞內(nèi)皮細(xì)胞、平滑肌細(xì)胞和一些轉(zhuǎn)化細(xì)胞產(chǎn)生。自然發(fā)生的TNF-α形式是糖基化的，但非糖基化的重組TNF-α具有相當(dāng)?shù)纳锘钚浴?jù)報道，TNF-α的生物活性原生形式是一個三聚體。人類和鼠類的TNF-α在氨基酸水平上顯示出大約79%的同源性，并且這兩個物種之間有交叉反應(yīng)。

|   特異性

可檢測樣本中大鼠的TNF-α，且與其類似物無明顯交叉反應(yīng)。

|   典型數(shù)據(jù)及參考曲線

由于實驗操作條件的不同（ 如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等），標(biāo)準(zhǔn)曲線的OD值會有所差異。以下數(shù)據(jù)和曲線僅供參考，實驗者需根據(jù)自己的實驗建立標(biāo)準(zhǔn)曲線。

注意：本圖僅供參考，應(yīng)以同次試驗標(biāo)準(zhǔn)品所繪標(biāo)準(zhǔn)曲線計算標(biāo)本含量。

|   重復(fù)性

板內(nèi)變異系數(shù)小于10%，板間變異系數(shù)小于10%

|   回收率

在選取的健康大鼠血清、血漿、細(xì)胞培養(yǎng)上清中加入3個不同濃度水平的大鼠TNF-α，計算回收率

|   線性稀釋

分別在選取的4份健康大鼠血清、血漿、細(xì)胞培養(yǎng)上清中加入高濃度大鼠TNF-α，在標(biāo)準(zhǔn)曲線動力學(xué)范圍內(nèi)進(jìn)行稀釋，評估線性。

注：ELISA試劑盒檢測范圍等數(shù)據(jù)，因檢測樣本 的不同而調(diào)整，實際數(shù)據(jù)以隨貨說明書為準(zhǔn)。

|   ELISA操作視頻

|   ELISA操作前必讀 / 下載

|   客戶發(fā)表的文獻(xiàn)

Photocurrent‐Directed Immunoregulation Accelerates Osseointegration through Activating Calcium Influx in Macrophages

Rank: IF 18.5

Journal: Advanced Functional Materials

Year: 2024

DOI: 10.1002/adfm.202406095

Magnetic Field Driven Ceria Nanosystems for Mitochondria Targeted Therapy of Ischemic Stroke

Rank: IF 18.5

Journal: Advanced Functional Materials

Year: 2025

DOI: 10.1002/adfm.202423291

Remodeling Tumor Immunogenicity with Dual-Activatable Binary CRISPR Nanomedicine for Cancer Immunotherapy

Rank: IF 15.8

Journal: ACS Nano

Year: 2023

DOI: 10.1021/acsnano.2c12107

Supramolecular Integration of Multifunctional Nanomaterial by Mannose-Decorated Azocalixarene with Ginsenoside Rb1 for Synergistic Therapy of Rheumatoid Arthritis

Rank: IF 15.8

Journal: ACS Nano

Year: 2023

DOI: 10.1021/acsnano.3c09140

Benefcial efect of the short-chain fatty acid propionate on vascular calcifcation through intestinal microbiota remodelling

Rank: IF 13.8

Journal: Microbiome

Year: 2022

DOI: 10.1186/s40168-022-01390-0

Transformable peptide blocks NF-κB/IκBα pathway through targeted coating IκBα against rheumatoid arthritis

Rank: IF 12.8

Journal: Biomaterials

Year: 2024

DOI: 10.1016/j.biomaterials.2024.122839

Volatile organic compound exposure in relation to lung cancer: Insights into mechanisms of action through metabolomics

Rank: IF 12.2

Journal: Journal of Hazardous Materials

Year: 2024

DOI: 10.1016/j.jhazmat.2024.135856

Complex Probiotics Supplementation Improves Overtraining-Induced Muscle Inflammatory Response and Antioxidant Status Via LPS-TLR4/NFκb Signalling Pathway in Rats

Rank: IF 11.4

Journal: Clinical Cancer Research

Year: 2021

DOI: 10.21203/rs.3.rs-513418/v1

Sphingosine-1-Phosphate Receptor Targeted PLGA Nanobubbles for Inflammatory Vascular Endothelial Cell Catching

Rank: IF 10

Journal: Advanced Healthcare Materials

Year: 2023

DOI: 10.1002/adhm.202301407

Tannic acid/Sr2+-coated silk/graphene oxide-based meniscus scaffold with anti-inflammatory and anti-ROS functions for cartilage protection and delaying osteoarthritis

Rank: IF 9.4

Journal: Acta Biomaterialia

Year: 2021

DOI: 10.1016/j.actbio.2021.02.046