|   別稱

IFN-G; IFG; IFI; INFr; IFN, Immune Interferon

|   IFN-γ 簡介

干擾素（IFN-γ，又稱II型干擾素或免疫干擾素）是一種主要由T淋巴細胞和自然殺傷細胞產(chǎn)生的細胞因子。該蛋白與IFN-β或各種IFN-α家族蛋白沒有明顯的同源性。成熟的IFN-γ以非共價連接的同質(zhì)體存在。IFN-γ最初是基于其抗病毒活性而被定性的。該蛋白還發(fā)揮抗增殖、免疫調(diào)節(jié)和促進炎癥的活性，因此在宿主防御機制中很重要。IFN-γ誘導(dǎo)細胞因子的產(chǎn)生，上調(diào)I類和II類MHC抗原、Fc受體和白細胞粘附分子的表達。它調(diào)節(jié)巨噬細胞的效應(yīng)功能，影響異型轉(zhuǎn)換并增強B細胞分泌免疫球蛋白的能力。IFN-γ還能增強TH1細胞的擴增，并可能是TH1細胞分化所必需的。

|   特異性

可檢測樣本中大鼠的IFN-γ，且與其類似物無明顯交叉反應(yīng)。

|   典型數(shù)據(jù)及參考曲線

由于實驗操作條件的不同（ 如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等），標(biāo)準(zhǔn)曲線的OD值會有所差異。以下數(shù)據(jù)和曲線僅供參考，實驗者需根據(jù)自己的實驗建立標(biāo)準(zhǔn)曲線。

注意：本圖僅供參考，應(yīng)以同次試驗標(biāo)準(zhǔn)品所繪標(biāo)準(zhǔn)曲線計算標(biāo)本含量。

|   重復(fù)性

板內(nèi)變異系數(shù)小于10%，板間變異系數(shù)小于10%

|   回收率

在選取的健康大鼠血清、血漿、細胞培養(yǎng)上清中加入3個不同濃度水平的大鼠IFN-γ，計算回收率

|   線性稀釋

分別在選取的4份健康大鼠血清、血漿、細胞培養(yǎng)上清中加入高濃度大鼠IFN-γ，在標(biāo)準(zhǔn)曲線動力學(xué)范圍內(nèi)進行稀釋，評估線性。

注：ELISA試劑盒檢測范圍等數(shù)據(jù)，因檢測樣本 的不同而調(diào)整，實際數(shù)據(jù)以隨貨說明書為準(zhǔn)。

|   ELISA操作視頻

|   ELISA操作前必讀 / 下載

|   客戶發(fā)表的文獻

Novel phloroglucinol derivative Compound 21 protects experimental autoimmune encephalomyelitis rats via inhibiting Th1/Th17 cell infiltration

Rank: IF 8.8

Journal: Brain Behavior and Immunity

Year: 2020

DOI: 10.1016/j.bbi.2020.03.009

The inhibition of pectin oligosaccharides on degranulation of RBL-2H3 cells from apple pectin with high hydrostatic pressure assisted enzyme treatment

Rank: IF 8.5

Journal: Food Chemistry

Year: 2021

DOI: 10.1016/j.foodchem.2021.131097

Effect of gestational and lactational nonylphenol exposure on airway inflammation in ovalbumin-induced asthmatic rat pups

Rank: IF 8.1

Journal: Chemosphere

Year: 2020

DOI: 10.1016/j.chemosphere.2020.126244

Se-rich tea polysaccharide extracted by high hydrostatic pressure attenuated anaphylaxis by improving gut microbiota and metabolic regulation

Rank: IF 7.7

Journal: International Journal of Biological Macromolecules

Year: 2024

DOI: 10.1016/j.ijbiomac.2024.132128

Prednisone acetate modulates Th1/Th2 and Th17/Treg cell homeostasis in experimental autoimmune uveitis via orchestrating the Notch signaling pathway

Rank: IF 4.8

Journal: International Immunopharmacology

Year: 2023

DOI: 10.1016/j.intimp.2023.109809

Sea Buckthorn Flavonoid Extracted by High Hydrostatic Pressure Inhibited IgE-Stimulated Mast Cell Activation through the Mitogen-Activated Protein Kinase Signaling Pathway

Rank: IF 4.7

Journal: Foods

Year: 2024

DOI: 10.3390/foods13040560

Preparation of corn glycopeptides and evaluation of their antagonistic effects on alcohol-induced liver injury in rats

Rank: IF 3.8

Journal: Journal of Functional Foods

Year: 2020

DOI: 10.1016/j.jff.2019.103776

Pediococcus acidilactici AS185 attenuates early atherosclerosis development through inhibition of lipid regulation and inflammation in rats

Rank: IF 3.8

Journal: Journal of Functional Foods

Year: 2019

DOI: 10.1016/j.jff.2019.103424

Acorus tatarinowii oils exert protective effects on microglia-mediated inflammatory injury via restoring gut microbiota composition in experimental stroke rats

Rank: IF 3.5

Journal: Brain Research Bulletin

Year: 2024

DOI: 10.1016/j.brainresbull.2024.110990

PTEN inhibitor attenuates cardiac fibrosis by regulating the M2 macrophage phenotype via the PI3K/AKT/TGF-β/Smad 2/3 signaling pathway

Rank: IF 3.2

Journal: International Journal of Cardiology

Year: 2022

DOI: 10.1016/j.ijcard.2022.04.007